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qbase relative quantification analysis software  (Biogazelle)

 
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    Structured Review

    Biogazelle qbase relative quantification analysis software
    Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
    Qbase Relative Quantification Analysis Software, supplied by Biogazelle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qbase+relative+quantification+software/qbase+relative+quantification+software/pmc04771800-199-51-48
    Average 90 stars, based on 1 article reviews
    qbase relative quantification analysis software - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Development of an experimental model of maternal allergic asthma during pregnancy"

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

    Journal: The Journal of Physiology

    doi: 10.1113/JP270752

    Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
    Figure Legend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Techniques Used: Staining, Membrane, Control

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Quantitative Proteomics:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Software:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Ubiquitin Proteomics:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Expressing:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Real-time Polymerase Chain Reaction:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Quantitative RT-PCR:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Modification:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Gene Expression:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    T-Test:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer

    Western Blot:

    Article Title: Metabolic and genetic responses to simulated overwintering conditions of alfalfa-rhizobia associations contrasted in their freezing tolerance
    Article Snippet: rmalized in each tissues with three reference genes: ubiquitin 5 (crown), eukariotic elongation factor 1-alphas (roots) and H-ATPase (crowns and roots). Relative quantification was calculated with the qBase software using the 2-ΔΔCq or comparative Cq method based on the differences in Cq between the target and the reference genes and corrected for PCR efficiency. Statistical analysis of biomass wer



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    Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
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    qbase relative quantification software  (Biogazelle)
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    Image <t>analysis</t> of Masson's trichrome‐stained lung sections for the <t>quantification</t> of airway smooth muscle (ASM; C) and collagen content (D) <t>relative</t> to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).
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    Image Search Results


    Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Journal: The Journal of Physiology

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

    doi: 10.1113/JP270752

    Figure Lengend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Article Snippet: The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    Techniques: Staining, Membrane, Control